The effect of DNA aptamer configuration on the sensitivity of detection thrombin at surface by acoustic method.
نویسندگان
چکیده
Using an acoustic method we showed that the aptamer configuration, especially those comprised of dimers with binding exosites sensitive to heparin and fibrinogen at thrombin, substantially increases the aptasensor sensitivity.
منابع مشابه
Designing a Label Free Aptasensor for Detection of Methamphetamine
A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...
متن کاملDNA Nanotubes Coupled with Magnetic Nanoparticles as a Platform for Colorimetric Biosensors
This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the second one was DNTs arayed with MNPs on their extrior surfaces. Then the both forms of MDNTs were employed as platforms for attaching a specifi...
متن کاملPotential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...
متن کاملA colorimetric aptasensor for selective detection of oxytetracycline in milk, using gold nanoparticles and oxytetracline-short aptamer
Objective (s): In light of misuse of antibiotics in animal husbandry and their side effects on human health, there is an argent need to develop simple and rapid methods for determining the quantification of antibiotics in biological systems. Materials and Methods: In this work a facile and ultrasensitive colorimetric aptasensor was reported for detection of oxytetracycline (OTC) in water and mi...
متن کاملA novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes
Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 41 شماره
صفحات -
تاریخ انتشار 2009